| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
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| Autores: |
Ben Amor, Faten
Elleuch, Fatma
Ben Hlima, Hajer
Garnier, Matthieu
Saint-jean, Bruno
Barkallah, Mohamed
Pichon, Chantal
Abdelkafi, Slim
Fendri, Imen
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| Data: |
2017-09
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| Ano: |
2017
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| Palavras-chave: |
Proteome
Dunaliella
18S rRNA gene
Carotenoids
Bidimensional gel electrophoresis
Mass spectrometry
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| Resumo: |
The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00402/51398/51973.pdf
DOI:10.3390/md15090293
http://archimer.ifremer.fr/doc/00402/51398/
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| Editor: |
Mdpi Ag
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| Formato: |
application/pdf
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| Fonte: |
Marine Drugs (1660-3397) (Mdpi Ag), 2017-09 , Vol. 15 , N. 9 , P. 293 (1-16)
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| Direitos: |
2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
info:eu-repo/semantics/openAccess
restricted use
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